Combination with Tadalafil reduces the aphrodisiac activity of Cola acuminata and Garcinia cola in rats

Abstract

Statistically, male infertility is increasing in almost every part of the world. Modern medicine provides nutritional, physiological and psychopharmacological treatment, however, many of them produce negative side effects. LD₅₀ of Cola acuminata was estimated to be 1095.44mg/kg, while that of Garcinia kola was above 5000mg/kg. Fifty male rats were randomized into 10 groups (A-J). Groups A and B served as controls with A receiving distilled water and Tadalafil (5 mg/kg). Groups C-E were administered 100 mg/kg, 200 mg/kg and 300 mg/kg Cola acuminata aqueous extract, respectively and groups F-H received 100mg/kg, 300 mg/kg and 500 mg/kg of Garcinia kola aqueous extract respectively. On the other hand, Groups I and J received a combination of Tardalafil, with aqueous extract of Cola acuminata, and Garcinia kola respectively. Tadalafil and the extract were administered via the oral route. Sexual behavioural parameters of mount latency, mount frequency, genital grooming, anogenital sniffing, and chasing of female animals were recorded in male rats (one hour after administration of extract) by their interaction with a receptive female (1:1) for an observatory period of 1 hour. Cola acuminata and Garcinia kola showed a dose dependent effect on the sexual behaviours studied. The aphrodisiac properties of the extracts of Cola acuminata and Garcinia kola at appropriate doses can be described as comparable to that of Tadalafil. However, the combination their combinations with Tadalafil showed an attenuation of the aphrodisiac activities of the plants extract.

Keywords: Acute toxicity, Aphrodisiac, Cola acuminata, Garcinia kola, LD₅₀, Tadalafil