Evaluation of Bioactive Agents in Calotropis Procera Plant Parts Through Anti-Microbial, Proximate, and Antioxidant Studies.

Abstract

Successive extraction of the aerial plant parts of Calotropis procera using soxhlet extraction yielded the phytochemicals; saponins, alkaloid, tannins, phenol, flavonoids, anthraquinones, and glycosides. Bioassay analysis using standard procedures like Mueller Hinton dilution experiment, demonstrated antibacterial potentials against microbes. E. coli, C. albicans, S. aureus, S. pyogenes, P. aeruginosa, S. typhi, and C. stellatoidea. 27 mm to 41 mm indicated  zones of inhibition range. The MBC and MFC for all the microorganisms were at 25 mg/ml except for S. pyogenes which was at 12.5 mg/ml. The MIC for the most sensitive organisms was 12.5 mg/ml with the exception of P. aeruginosa and S. pyogenes which had theirs at 6.25 mg/ml. Proximate analysis used standard AOAC method, and in antioxidant analysis, free radical scavenging method of 2, 2-diphenyl-1-picryl-hydrazyl (DPPH) were employed. The results are as follows, proximate analysis revealed the percentage contents of fat, crude fiber, protein, ash, and carbohydrates to be 2.93, 7.05, 1.68, 56.50, and 18.69, respectively. In the antioxidant studies, free radical scavenging technique employing 2, 2-diphenyl-1-picryl-hydrazyl (DPPH) was used. Samples and standards were determined using the IC50. The active sample was hexane extract, with an IC50 of 0.0878, followed by ethyl acetate (0.8811) and methanol (0.9215).