Isolation and Characterization of Bioactive Constituents of Boscia salicifolia. Olive (Capparaceae)

Abstract

Extraction of the crude extract of B. salicifolia was carried out using methanol as solvent with the aid of Soxhlet apparatus. The extract was concentrated in-vacuo at 40^oC using rotary evaporator. The crude methanol extract was subjected to thin layer chromatography and vacuum liquid chromatography (VLC). The isolated compound BSME was characterized by IR and GC-MS. The IR spectral studies indicated the presence of hydroxyl, carbonyl, alkane and aromatic functional groups. GC-MS revealed nine prominent peaks with retention times of 5.133, 11.62, 14.692, 15.725, 16.475, 17.433, 18.183, 18.317 and 20.733 minutes indicating the presence of 1,2,3-propanetriol, 3,5-diterbutylphenol, 2-(2-hydroxy-2-phenylethoxy)phenol, methyl-12-methyltetradecanoate, n-Hexadecanioc acid, 13-octadecanoate, 9- Octadecanioc acid, n- Octadecanoic acid and 9- octadecenal respectively.