Effect of Honey on Oxidative Stress in the Brain Tissue of Sleep Deprived Rats

Abstract

This study aimed to demonstrate the effect of honey on the oxidative stress in the brain of sleep-deprived rats. Twenty five 6 weeks old male wistar rats were randomly divided into five groups and subjected to paradoxical sleep deprivation and recovery for 5 days using the modified multiple platform (MMP) method. Group I: Normal control; Group II: sleep deprivation (SD); Group III: sleep deprivation and sleep recovery  (SD+SR) – received 10 ml/kg distilled water orally each; while Group IV: sleep deprivation and honey (SD+Honey) and Group V: sleep deprivation, recovery with honey (SD+SR+Honey) received honey (1g/kg body weight) orally once daily. Brain tissue of the humanely sacrificed rats were excised and homogenized for assessment of oxidative stress markers. Results indicate a significant decrease (p<0.05) in the concentrations of malondialdehyde (MDA) in brain tissues of rats in Groups IV and V when compared with that from sleep deprived group (Group II). The Increase in reduced glutathione (GSH) concentration and catalase enzyme activity observed in homogenized brain tissue of rats in Groups III, IV, and V differ significantly (p<0.05) when compared with Group II. The results suggest that treatment with honey probably has ameliorative effects on oxidative stress in brain tissue of sleep deprived experimental rats.