In-Vitro Growth Enhancement of Cedrela odorata L. using Benzyl Amino Purine and Α-Naphthalene Acetic Acid

Abstract

Studies were conducted in order to develop protocol for mass-production of Cedrela odorata seedlings. Clean-plantlets were initiated on four media at full and half strengths. Shoot-regeneration consisted two media types (Murashige & Skoog (MS) and Woody Plant Medium (WPM)) and four Benzyl Amino Purine (BAP) concentrations: 0.0, 1.0, 2.0 and 3.0 mg/l. Root-induction involved three MS media strengths (25, 50 and 100 % basal salts) and two Naphthalene Acetic Acid (NAA) levels (0.0 and 0.5 mg/l) all in factorial combinations and laid out in completely randomised design (CRD). The results at initiation showed that MS at full strength enhanced in vitro seeds growth better in terms of root lengths (6.50 cm) and number of leaves (7.6) while WPM gave better shoot length (6.24 cm) at 3 Week after inoculation (WAI). Shoots were best regenerated on MS basal medium supplemented with 1.0 mg/l BAP for shoot length at 4 WAI while the effects of BAP levels were comparable but higher than control for number of leaves and axillary shoots. Highest number of roots (3.92) induced and root length (2.93 cm) obtained were from 100% MS medium at 8 WAI. Hence, MS medium (100% basal salt) with 1.0 mg/l BAP with or without 0.5 mg/l NAA are considered suitable protocol for in vitro propagation of C. odorata.