Screening and Selection of Cryptococcus Neoformans Using Urease Activity and Temperature Range

Abstract

Pigeon dropping has been known to be a reservoir for Cryptococcus neoformans. In this research, attempt has been made to screen and select for this life threatening organism using temperature (thermotolerance) and urease test sequentially. Fourteen (14) samples from pigeon droppings were collected from three markets in Benin City. Isolates obtained from these samples were subjected to urease and temperature test. The results showed that 10 isolates that were positive for temperature were equally positive for the urease tests, with highest growth rate 37°C recorded for samples obtained from New Benin (NB3). This experiment demonstrates the possibility of isolating C. neoformans at minimum temperature of 37°C thermotolerance and urease activity tests. Pathogenicity studies are recommended to further understand host – pathogen relationships.

Keywords: Cryptococcus neoformans, Pathogenic, Urease, Thermotolerance, Isolates