Cellulase production by Proteus spp. JC402 from plantain fruits stalk biomass using submerged fermentation

Abstract

Biodegradation of lignocellulosic biomass into a variety of value - added products (monomeric sugars) by the action of cellulolytic enzymes has recorded a significant value for industrial processes. This present work aimed at isolating and screening bacteria of soil origin capable of producing extracellular cellulase, and investigated hydrolysis of plantain fruit stalk biomass (PFSB) using submerged fermentation. Alkaline pretreated and untreated PFSB substrates were used for the cellulase production. Enzyme extract was assayed using carboxyl methyl cellulose assay method. Out of eight bacteria species screened for cellulase producing potential, four showed positive on screening medium indicated by clear or halo zone around growing microbial colonies. Proteus spp. JC402 was selected based on highest zone of inhibition (23 mm) on screening medium and 100% hydrolysis efficiency. Cellulase activity of pretreated PFSB 7.5% (w/v) substrates has the highest activity 3.2 ± 0.14 Uml-1 and untreated PFSB 2 % (w/v) has 2.76 ± 0.31 Uml-1 at 48 h. Cheap, unexploited, renewable and readily available agricultural cellulosic biomass has been effectively utilized for the production of a thermo stable enzyme as criteria for industrial application and environmental implications.