Main Article Content

Purification and Characterization of Lipase from Aspergillus flavus PW2961 using Magnetic Nanoparticles.


S.O. Kareem
O.S. Adebayo
S.A. Balogun
A.I. Adeogun
S.B Akinde

Abstract

Lipase from Aspergillus flavus was purified in a single step purification using MnFeO4 magnetic nano particles to achieve a 20.53- fold purification with specific activity of 11.29 U/mg and a 59% recovery yield. SDS-PAGE of lipase showed a single pure band with corresponding molecular weight of 35 kDa. The optimal temperature and pH for the enzyme activity were 45°C and 7.0 respectively. Addition of olive oil (1 %w/v) enhanced pH stability of the lipase with 86% residual activity at pH 7.0 after 6 h of incubation while the lipase was thermostable with 79% residual activity after 4 h of incubation. The enzyme activity was enhanced by Ca2+, Mg2+ and Mn2+ while the presence of Cu2+, Co2+ and Zn2+ exhibited inhibitory effect on the enzyme. The study therefore presented lipase from Aspergillus flavus PW2961 with potential in industrial and biotechnological application.

Key words: Lipase, purification, Aspergillus flavus PW2961, magnetic nanoparticles


Journal Identifiers


eISSN:
print ISSN: 0189-1731