Influence of Manganese on Ochratoxin A Detoxification in Rats

Abstract

The Influence of manganese on ochratoxin A detoxification was studied in 3- month old female white albino rats administered with 0.00, 500 and 500μg/kg body weight of ochratoxin A intraperitoneally daily for five days. In addition to the ochratoxin A, 0.5ml of 5% Manganese (II) chloride was administered to one of the test groups by intubation. The rats were monitored for mortality and clinical signs for five days before sacrifice. Serum samples were assayed for liver enzymes and kidney biomarkers. Histopathological examinations of the liver and kidney were conducted. The results showed that the administration of 500μg/kg body of ochratoxin A caused injury to the liver and kidney of the test groups though it was milder in the group given manganese and there was no mortality recorded. There was a significant increase (P< 0.05) in activities of the enzymes aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Alkaline Phosphatase (ALP) in the test groups, while it was a non significant (P > 0.05) increase for creatinine level. Histopathological examinations showed degeneration of portal nuclei , walls of porteries and veins, pericholongitis, congestion of portal vessels by white blood cells, hepatocellular necrosis and perivascular cuffing in the liver and lymphocytic infiltration, haematin fragments, edema and tubular necrosis in the kidney of the test groups only. This study indicates clearly that manganese no significant influence on ochratoxin A detoxification.

Key Words: Ochratoxin A, Biochemical, Histological, Detoxification, Manganese, Rats.