Reduction of Exudates (Browning) in Sugarcane Micro Propagation

Abstract

Ex plant preparation in the course of sugarcane micro propagation is known to release exudates which lead to the death of sugarcane in vitro. An industrial sugarcane variety (NCS008) was used for the study and was observed to die in less than 14 days in vitro. In a study to reduce the death of this variety, ascorbic acid and citric acid was added as constituent of the media using MS and stock at different concentrations. The browning was reduced drastically at the addition of 0.1g/litre ascorbic acid and 0.15g/litre citric acid.

Keywords: reduction, exudates browning, micro propagation, sugarcane

Nig J. Biotech. Vol. 23 (2011) 40 - 44