In-vivo Effect of Oral Administration of Bifidobacterium adolescentis CH2 of Avian Origin on Male Albino Rats

Abstract

Before administering to humans and agricultural animals, probiotics must satisfy  requirements for safety. Bifidobacterium adolescentis CH2 isolated from chicken was orally administered to male albino rats at a daily dose of about 3.6 x 106 cfu for 4 weeks to investigate the safety of its oral dose effects. Oral ingestion impact was assessed by body weight, blood biochemical components analysis, liver and kidney histopatological evaluations. Results from parameters investigated indicate no  adverse effect of ingestion of B. adolescentis CH2 strain when the dosed group is compared with the control group. No mortality, abnormal weight, organ injury or damage was observed in the control and B. adolescentis CH2 administered group. There was no abnormal decrease or increase in creatinine, aspartate transaminase, urea and blood glucose in any group. Histopatological examination of liver and kidney showed no abnormality or damage to organ in all groups. B. adolescentis CH2 strain isolated from chicken had no adverse effect on albino rats with respect to parameters here studied.

Keywords: Bifidobacterium adolescentis, probiotic, in-vivo safety, yoghurt.