Biocontrol Of Viral Necrotic Disease Of Phaseolus Vulgaris By Rhizosphere Microorganisms

Abstract

This study revealed that the preinoculation of soil with rhizosphere microorganisms significantly reduced number of local lesions produced by TNV, in P. vulgaris plants either grown in amended or unamended soil. Phaseolus plants grown in fish meal amended soil supplied with RMs singly or in mixtures, and then their leaves sprayed with RMs metabolites for different intervals (1, 3, 7, 10) days, such treatment induced de novo proteins in treated and distant untreated leaves. Electrophoretic analysis on 15% PAGE confirmed the induction of novel proteins with all treatments. However some variations were recorded in the number and molecular weight of separated bands. The number of new protein bands was ranged between 1-6 bands. The apparent molecular weights of new bands were ranging between 15 to 180 KDa.

New Egyptian Journal of Microbiology Vol. 17 (2) 2007: pp. 247-261