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Dec 31, 2022Keywords:
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Main Article Content
Influence of Basal Media Variants on the Accumulation of Phytochemicals in Leaves of Moringa Oleifera Lam. In Vitro
Abstract
Moringa oleifera Lam. is renowned for its medicinal and nutritional values due to the presence of various phytochemicals and nutrients which are beneficial to man. This study compared the influence of three standard plant tissue culture basal media, namely: Murashige and Skoog medium, Gamborg B5 medium and White medium, on the accumulation of some secondary metabolites in the leaves of Moringa oleifera. The explants were pre-sterilized and the sterile seeds were transferred to the culture tubes containing the different basal media and kept in a growth room for four weeks. The leaves from the treatments were harvested and air dried for the phytochemical studies. Agar and water served as the control. The experimental design was Completely Randomized Design (CRD). Data were subjected to Analysis of Variance (ANOVA) and means were separated using Duncan’s New Multiple Range Test (DNMRT) at (P ≤ 0.05). All the treatments supported the production of phytochemicals; however there were significant differences among the treatments in some of the parameters studied. B5 medium best enhanced the production of tannin (0.038 ± 0.00 %), alkaloids (25.00 ± 0.11 %), glycoside (4.75 ± 0.25 %) and carotenoids (80.00 ± 0.00 %), while the White medium enhanced the production of flavonoid (22.00 ± 2.00 %) when compared to other treatments (P ≤ 0.05). This study provides useful information that could be exploited for the production of phytochemicals from M. oleifera seeds in vitro.
