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PERFORMANCE OF COFFEE LEAF EXPLANTS IN SOMATIC EMBRYOGENESIS
Abstract
Explants of four coffee genotypes: T169,C111, C314 and D57 were cultured and maintained at 27±2oC for 14 to 42 days. Calli observed were then transferred to the embryo development (ED) medium and incubated for 90 days, Matured calli were then cultured in appropriate growth media to generate secondary somatic embryos. Varying concentrations of casein hydrolysate in 1000ml ED medium were thereafter separately tested against the resulting six-leaf explants of each of the clones. Overall results showed that clones C111, C314 and T169 responded well to embryogenesis while D57 did not. Techniques used in this study can therefore be used to develop somatic embryos for the micropropagation of some Nigerian premium coffee genotypes.
