Molecular detection and serotyping of foot and mouth disease virus in tongue epithelium samples

Abstract

The aim of the present study is molecular identification and typing of FMDV circulating in Dakahlia governorate in Egypt, 2014. The study was accomplished on pooled nine tongue epithelium samples collected from diseased cattle and buffaloes expected to be infected with FMDV. The diseased animals showed fever , excessive salivation and ulceration on tongue and gum. Conventional reverse transcription polymerase chain reaction (RT-PCR) was used for identification of FMDV using universal primers. Then serotyping of the identified virus was done using RT-PCR for serotype O and A and Real time RT-PCR for serotype SAT2 . FMDV was identified in all the examined nine samples. Five of them were of serotype O, two of serotype A, one of serotype SAT2 and one sample was neither O, A nor SAT2. In conclusion, molecular techniques are sensitive and rapid methods for FMDV detection. In addition, serotypes O, A and SAT2 are circulating in Dakahlia Governorate, Egypt, 2014.