Identification of single nucleotide polymorphisms in exon 2 of Bone Morphogenetic Protein 15 gene in Egyptian buffaloes

Abstract

Bone Morphogenetic Protein 15 (BMP 15) is a member of transforming growth factor-β (TGF- β) superfamily which plays an important role in ovarian follicular development and ovulation rate. In this study a partial sequence of this gene was screened for polymorphisms. PCR amplification of DNA isolated from blood of 100 Egyptian buffaloes hiefers (50 infertile and 50 normal). Revealed two loci, BMP15.1 (222 bp) and BMP15.2 (222 bp) which includes exon 2 of BMP 15 gene. Polymorphisms in these two loci were detected by using direct sequencing and single strand conformational polymorphism (SSCP) technique. A single pattern of SSCP was detected. This was confirmed by nucleotide sequencing which revealed absence of single nucleotides polymorphisms (SNPs) or any other type of polymorphisms in the two loci. The sequencing and PCR-SSCP patterns revealed no polymorphism in all studied animals. Further studies must be done on another loci on BMP 15 gene or other genes related to infertility in Egyptian buffaloes.