Histopathological study on the effect of Artemisinin multiple doses on the pathogenesis and immune reaction of Trichinella spiralis in albino rats

Abstract

Objective: The aim of this study was to determine the therapeutic effect of Artemisinin (AR) multiple doses on the pathogenesis and  immune reaction of (T. spiralis) during the intestinal and muscular phases in albino rats.

Design: Randomized controlled study.

Animal Models: The experiment was performed on 80 adult male and female albino rats weighting (160 g ± 20 g) and their age ranged  from (4-6) weeks.

Procedures: rats were divided into four experimental groups; 20 rats/each. 1-(Control negative) group. 2- T. spiralis infested (TR) group:  each animal was orally infested with 300 T. spiralis larvae. 3- T. spiralis treated (AR+TR) group: each animal in this group was orally infested  with 300 T. spiralis larvae followed by treatment with AR at dose of (400 mg / kg body weight) one day post infection (PI) for three successive days and other three successive doses on days 21, 22 and 23 PI. 4-(AR) group, animals received AR only at the same dose.

Results: The current study recorded that AR administration showed improvement of the intestinal histopathological,  morphohistopathological analysis and ultra-structural alterations during the intestinal phase. In addition to its efficacy in decreasing the  larval count during the muscular phase. However minimal improvement in the histopathological and ultra-structural alterations of the  infected skeletal muscles were observed. The findings of muscular morphohistopathological analysis, biochemical and hematological  analysis were not improved.

Conclusion and clinical relevance: AR had significant anthelminthic efficacy against the adult stage of T.  spiralis during the intestinal phase and minimal efficacy against encysted muscle larvae