Study of the biological activities of the seeds of the plant Ceratonia siliqua/i L. recovered in the Bejaia region

Abstract

Background: Ceratonia siliqua is a plant that belongs to the Fabaceae family. It is frequently used in our culinary and medical traditions to fight cholesterol, acute diarrhea and digestive disorders.
Methods: The hydrogen atom or electron donation abilities of the corresponding extracts and some pure compounds were measured from the bleaching of the purple-colored methanol solution of 2, 20- diphenylpicrylhydrazyl (DPPH), studying the anti-inflammatory effect by measuring the volume of the edema of the paw that has received carrageenan 1%. Antimicrobial activity in vitro was screened by using disc diffusion and micro-dilution methods.
Results: The determination of the polyphenols of the methanolic extracts of the seeds reveals the richness of our extracts in polyphenols is 30 mg of gallic acid / g of extract. The aqueous and methanolic extract flavonoid assay shows that they contain high levels of flavonoids with contents of 07 and 10 mg equivalent of quercetin / g of extract respectively. The aqueous and methanolic extracts of Ceratonia siliqua seeds from the Bejaia region could bring the stable free radical 2.2 diphenyl-1-picrylhydrazyl (DPPH) to yellow-colored diphenylpicrylhydrazine with respective IC 50 values of 0.7 mg/ml and 0.2 mg/ml. They exhibit lower antioxidant activity than ascorbic acid (0.038 μg / ml). Determination of anti-inflammatory activity revealed that our aqueous and methanolic extracts of Ceratonia siliqua L seeds are able to reduce edema up to 81.89%. The methanoid extracts of Ceratonia siliqua L. seeds have a medium antibacterial action against bacteria: E. coli (13 mm), Staphylococcus aureus (12 mm) and Pseudomonas aeruginosa (12 mm). On the other hand, the aqueous extracts of the seeds of Ceratonia siliqua L have a weak antibacterial action against the same bacteria with a diameter of inhibition of 9 mm. However, the aqueous extracts of Ceratonia siliqua L seeds are endowed with a very important inhibitory action against candida albicans and it is similar to that of the antibiotic, antifungal Econazole (1%).
Conclusion: According to the results observed, the two extracts of the seeds and pods have a potential anti-free radical and antioxidant measured compared to the standard antioxidant used. The results of the antimicrobial activity carried out in vitro on the 3 bacterial strains indicate that the two methanolic extracts of this plant have low antibacterial activity.