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The eff The effect of calcium hy ect of calcium hydroxide intr xide intracanal medicament on r acanal medicament on root dentine microhardness: An in vitro study
Abstract
Objective: To evaluate root dentine microhardness after using calcium hydroxide (Ca (OH)2) intracanal medicament.
Materials and methods: Forty single-rooted teeth were sectioned at the cementoenamel junction level, and the root canals were prepared till an apical size of #30. The prepared teeth were divided into two groups (n ¼ 20): the no medicament (control) group and the Ca (OH)2 group. After 7 days, medicaments were removed using a standardized volume of irrigation. All teeth were sectioned in a buccolingual plane and embedded in acrylic resin horizontally with the dentine surfaces exposed. Using Vickers microhardness tester with magnification of £ 100 and a load of 25 g for 10 s, a microhardness assessment was performed. Independent t test was used for intergroup comparison. One-way analysis of variance and post-hoc Tukey's tests were used for comparisons (P ≤ 0.05).
Results: Microhardness in the control group was significantly greater than in the Ca (OH)2 group at the coronal (P < 0.001) and middle (P ¼ 0.006) sections. At the apical third, no significant difference was detected (P ¼ 0.779). In the control group, the coronal section was significantly greater than at the apical section (P ¼ 0.002), while no significant difference was detected between the middle sections with both coronal (P ¼ 0.212) and apical (P ¼ 140) sections. In the Ca (OH)2 group, no significant effects were noted between different sections (P ¼ 0.385).
Conclusions: The Ca (OH)2 reduced root dentine microhardness at both coronal and middle sections, but not the apical.