Antibiotic sensitivity profile of bacteria isolated from urinary catheters in urinary tract infections’ patients

Abstract

Background: Urinary catheterization is a common procedure found in hospitals, and is even more common in intensive care units. There are risk factors associated with urinary tract infection (UTI), of which the use of a urinary catheter accounts for more than 80% of all health care associated UTIs. This study was conducted to determine the antibiotic susceptibility profile of bacteria associated with urinary catheters from UTI patients.

Methods: Urine samples were collected aseptically from a removed catheter within 24 hours of insertion and cultured on blood agar and Cysteine Lactose Electrolyte Deficient Agar (CLED) and identified. Antibiotic susceptibility test was conducted using disk diffusion method on Mueller Hinton agar.

Results: A total of 47 isolates were obtained, E. coli 15(31.92%), P. aeruginosa 4(8.51%), P. mirabilis 5(10.64%), P. vulgaris 5(10.64%), K. pneumonia 3(6.38%), S. aureus 11(23.40%), S. saprophyticus 4(8.51%) and showed various degrees of resistance and susceptibility to various antibiotics. E. coli was 73.3% and 66.7% resistance to gentamycin and cefotaxime respectively. P. aeruginosa showed 100% and 50% resistance to Nitrofurantoin and cefatoxine respectively, P. mirabilis showed 100% resistance to cefatoxine and 60% to gentamycin, nalidixic acid and tetracycline. P. vulgaris was also 100% resistance to gentamycin, ampicillin, cefotaxime and 80% to tetracycline. K. Pneumonia showed 100% resistance to tetracycline, ampicillin and norfloxacin. S. Saprophyticus showed 100% resistance to only teicoplanin. However, all the isolates were 100% susceptible to at least one antibiotic.

Conclusion: The bacterial pathogens associated with infection in urinary catheters in this study respond to different antibiotics at different degrees ranging from sensitive to resistant.