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Somatic Embryogenesis in Juniperus Procera using Juniperus Communis as a Model
Abstract
The aim for this particular research was initially an adaptation of optimum half strength lithium chloride-sodium propionate (LP) medium protocol for growth and proliferation of embryogenic culture. But as other conifer species it was difficult to stop proliferation of somatic embryo cell division and bring them to maturation stage. However using optimal concentration of ABA (32 mg/l), it was possible to get maturation response in most of the cultures.
Additional study on the effect of seed extraction to the growing embryogenic culture showed no effect on mature somatic embryos. Rather they were showing faster growth which increases embryogenic mass of each culture. It was also observed that green premature seeds of Juniperus are suitable for the initiation and proliferation of somatic embryo.
Key words: Cryopreservation, Juniperus communis, Juniperus procera, Somatic embryogenesis.
