Article Sidebar
Published:
Feb 6, 2025Keywords:
Article Details
The Momona Ethiopian Journal of Science accepts the manuscripts for consideration with the understanding that the manuscript has not been published and is not under consideration for publication elsewhere. Only original articles will be considered for publication if they have been published previously as abstracts, but not if they have been published previously as extended abstract (>1000 words). This applies to both electronic and print versions of the journal. The authors should assign copyright ownership to the Editorial Office of MEJS in the event that the manuscript is accepted for publication in the Momona Ethiopian Journal of Science. All accepted manuscripts must be accompanied by a copyright statement signed by all authors. A copy of the copyright form will be supplied along with the final reviewed version of the manuscript that is sent for final proof- reading. Authors may make multiple copies of the form if necessary and send to the Editorial Office with author’s signature(s) even individually.
All articles published by Momona Ethiopian Journal of Science (MEJS) are Open Access under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0). Under this license, authors retain ownership of the copyright for their content, and anyone can copy, distribute, or reuse articles as long as the author and original source are properly cited. In all these cases for re-use, the authors will be given proper credit to the original publication in MEJS.
Main Article Content
Gender-Based Disparities in Hepatitis B Virus X Gene Detection in the Patients Undergoing Antiviral Treatments
Neha Nadeem
Muhammad Zubair Yousaf
Abstract
The Hepatitis B Virus (HBV) is a 3.2 kb long virus belonging to the Hepadnavirus family. It has a variety of clinical symptoms, with chronic hepatitis like liver cirrhosis and hepatocellular cancer, based on immunological interactions between the virus and the host. Compared to the other genes of HBV, the X gene is highly conserved in the virus's genomic characteristics. Many mutations in the X gene of HBV can lead to the severity and other disease complications. This study was designed to determine the HBV X gene's detection in local patients' serum samples. Out of 40 collected samples from HBV patients, 24 (12 from each male and female patient) were identified as chronically HBV samples through various diagnostic approaches such as ICT rapid test, ELISA, and Real-time PCR. The samples used for DNA extraction yielded an excellent concentration of DNA ranging from 2.4ng/µl to 9.8 ng/µl. The HBV X gene-specific primers set showed results at 55°C for Nested PCR. The results were confirmed with gel electrophoresis. A band size of 597bp compared with the 1kb and 50bp DNA ladder was observed. The PCR-amplified products were purified and sent for sequencing. The sequencing results have significantly helped to analyze the sequences of the X gene (Consensus Sequence of local isolates) using bioinformatics tools like nBLAST, BioEdit, Expasy, MEGA11, and Phylogenetic analysis. The study indicated that despite taking antiviral treatment, the detection of the HBV X gene in chronically infected male patients is more than in female patients. The statistical analysis determined a significant difference (p<0.05) between the detection of the HBV X gene in males and females. In the future, this study will contribute to designing more specific assays and combined targeted therapies for Chronic HBV infection caused explicitly by mutations in the HBV X gene.
