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DOI:
10.4314/mejs.v11i2.4Keywords:
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Isolation and Antimicrobial Sensitivity Testing of Escherichia coli from Fish Meat Retailing Shops of Mekelle City, Ethiopia
Abstract
The present study was conducted for the period of six months, September, 2016 to February, 2017 so as to isolate and identify Escherichia (E.coli) from fresh fish samples collected from four different retailing shops in Mekelle city and determine the antibiotic resistance pattern of isolated bacteria. Isolation and identification of E.coli and antibiotic sensitivity test were performed following the standard microbiological techniques. The antibiotic resistance patterns of the E.coli isolates were tested against 6 different antibiotics, namely Ampicillin, Amoxicillin, Ciprofloxacin, Norfloxacin, Gentamicin and Streptomycin. Accordingly, out of the total 96 fish samples collected and analyzed, 9(9.4%) were positive for E.coli following the primary and secondary biochemical test results. Out of the four fish meat retailing shops, the highest and statistically significant prevalence (P=0.001) of E.coli was recorded in shop III as 5(20.8%) followed by shop II as 4(16.7%).Furthermore, results of the antimicrobial sensitivity test have shown that all of the isolates were completely significantly (P=0.001) resistant to two antibiotics (Ampicillin and Amoxicillin) and 100% significantly susceptible (P=0.001) to three of the antibiotics (Ciprofloxacin, Norfloxacin, and Gentamicin). Whereas, out of the 9 isolates, 2 isolates (22.2%) were intermediate and 7 isolates (77.8%) were susceptible to Streptomycin. Hence, it can be concluded that fishes from Mekelle shops do contain antimicrobial resistant pathogenic E.coli where the proportion of these isolates significantly (P<0.001) varies from shop to shop. In spite of the lesser practice of their utilization, Ciprofloxacin, Norfloxacin, and Gentamicin were found to be the best antimicrobials to treat E.coli associated infections in the study area. However, it is suggested that post-harvest hygienic management practices are to be practiced along the fish production-marketing chain.
Antibiotic sensitivity test, Escherichia coli, Fish, Mekelle, Retailing shops.
