Laboratory diagnosis and treatment of pathogenic bacteria isolated from children with cervical lymphadenopathy at Tanta University Hospital

Abstract

Immune illness, cancer, and microbial infections can cause lymphadenopathy, which enlarges lymph nodes. Current research focuses on laboratory diagnosis of microbial infections that cause lymphadenopathy in children by isolating and identifying microorganisms from throat and lymph node aspiration, performing throat and lymph node aspiration cultures, and treating the most common isolated bacterial infections with bioproducts. Children shown lymphadenopathy were microbiologically examined. Bacterial taxa recovered from cervical lymphadenopathy patients were Staphylococcus aureus (MRSA) and Streptococcus pyogenes. The two isolates were identified molecularly, and their sequences had been deposited in GenBank under the accession numbers PP527743 and PP527744 respectively. The agar well diffusion test assessed the antimicrobial properties of the clove, garlic, thyme oils and Aloe vera gel. Comparatively, garlic oil was the best antibacterial bioproduct against isolated bacteria, with inhibition zones of 20- and 21-mm. respectively. The minimum garlic inhibitory concentrations against MRSA and S. pyogenes were 6.25% and 0.19%. Cefotaxime antibiotic came first in comparison with other used antibiotics (Vancomycin, Ampicillin/sulbactam, Penicillin G) against common isolated bacteria and the mean diameter of inhibition zones were 31 mm and 35 mm, respectively. This study concluded that, the most common isolated bacterial infections from children with cervical lymphadenopathy were methicillin-resistant S. aureus and S. pyogenes. Among the several bioproducts tested against bacteria recovered from children with cervical lymphadenopathy, garlic oil demonstrated the highest antibacterial efficacy.