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The copyright of a submitted article is only transferred to the publishers if and when the article is accepted for publication. All rights reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means, electronic, electrostatic, mechanical, photocopying, recording or otherwise without the prior written permission of the publishers.
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Simultaneous Determination of Sulphamethoxazole and Trimethoprim in Co-Trimoxazole Tablets Using Rp-Lc Analysis
Abstract
A reversed phase liquid chromatographic (rp-lc) method has been developed to determine simultaneously the amount of sulphamethoxazole and trimethoprim in co-trimoxazole tablets using salicylic acid as internal standard. The chromatographic resolution was achieved with 96% ethanol and 0.1M ammonium perchlorate buffer (95:5) on a reversed phase column at ambient temperature. UV detection was at 254 nm. The flow through the column was 0.8ml/min with a chart recorder speed of 5mm/min. Mean retention times for trimethoprim sulphamethoxazole, and salicylic acid were 3.4 ± 0.13 min, 3.8 ± 0.08 min, and 4.5 ± 0.13 min respectively. Calibration curves were linear over the ranges 0.0001-0.0006%w/v (sulphamethoxazole) and 0.002-0.012%w/v (Trime-thoprim). The method has been used to analyze a branded co-trimoxazole (Septrin) tablets and a locally formulated co-trimoxazole tablets. The percentage content of sulphamethoxazole and trimethoprim determined by the rp-lc and the official u.v methods were found to be comparable and within the BP 2000 standards. Comparing this work with that of Rөnn et al 1999, revealed that, this method is cost effective for routine analytical work as the mobile phase is devoid of acetonitrile, an expensive reagent. Additionally, running time for the current method (4 min) is shorter than that of Rөnn et al (7 min), for in-process and finished product quality monitoring.
Journal of Science and Technology Vol. 26 (3) 2003: pp. 64-72
