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Effect of Carbon Source (Sucrose) on the Morphogenesis of Pineapple (Ananas Comosus (L) Merr) Cultured in-Vitro
Abstract
Sucrose is used in tissue culture as a source of carbon which is normally fixed by plants from the air. Some plants can make use of the physical environment and fix carbon (autotrophy) in tissue culture but such growth have been reported to be reportedly to be relatively slow. The level of sucrose in tissue culture can affect the growth and development of the plant. The type of specie of plant may react differently to the sucrose levels. The paper reports differently to the levels of sucrose and the effect of activated charcoal in the culture medium. The sucrose concentration was significantly affected the number of shoots regenerated. The mean shoots number regenerated at 3% level (8.20) was significantly different from either the 2.1 or no - sucrose medium. The extension of the original shoots was highest at the 3% level and lowest when no-sucrose medium and 61.7% than the 2% medium. Activated charcoal (AC) alone in the medium did not induce morphogenesis/organogenesis significantly in the absence of sucrose (in pineapple) but affected plant growth.
