Extraction of high-quality genomic DNA from mansonia altissima for microsatellite analysis

Abstract

This study was carried out to compare the deoxyribonucleic acid (DNA) extracted from fresh and dry leaves of Mansonia altissima with DNA extracted from fresh and dry cambium of the same species. Genomic DNA was extracted using Cetyltrimethylammonium bromide (CTAB) and DNA quantification was done using a UV Spectrometer. From the results obtained, fresh leaf and dried cambium samples had the highest DNA purity of 1.18 and 1.19 (at an absorbance ratio of A260/280) and 1.15 and 1.11 (at an absorbance ratio of A260/230), respectively. DNA obtained from dried leaf samples had the lowest purity. The samples from fresh leaf and dried cambium had the highest concentrations of 56.77μg/ml and 55.27μg/ml respectively, while samples from fresh cambium had the lowest concentration of 13.47μg/ml. Subsequently, samples from fresh leaf and dried cambium exhibited the highest yields of 2.84μg and 2.77μg respectively, with samples from fresh cambium having the lowest yield of 0.67μg. This research therefore showed that samples from dried cambium exhibited considerably high DNA concentration and yield, and hence recommended for use for further molecular studies of the high-canopy trees of Mansonia altissima.