Protocol development for in-vitro propagation of Anthocephalus cadamba

Abstract

The objective of this study is to establish the most suitable protocol for the micro propagation of Anthocephalus catamba. The seeds of the species were collected from the wild and stored in the Seed bank until time of use. These were subjected to surface-sterilization using systemic fungicide and other disinfectants. Different media strengths of Murashige and Skoog (MS) were used to determine the most efficient nutritional requirement. The media strengths employed in this experiment were ¼, ½ and full. The culture media were supplemented with 6-Benzylamino purine (BAP), Giberrellic acid (GA3) and N-acetic acid (NAA). The generated plantlets had no contamination in the growth room due to the methods employed in the disinfection. In almost all the results obtained, both the ¼ and ½ strength media produced better result than plantlets growing on full strength media. Treatment D (¼ strength, 0.1mg/L BAP, 0.2mg/L GA3 & 0.1mg/L produced the best results in all. From the results obtained from this study, it is recommended that lower basal salts will be required for the in-vitro propagation of A.cadamba.

Keywords: Anthocephalus cadamba, growth hormone, media strength, micro-propagation, plantlets