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Published:
Jan 27, 2005Keywords:
assay Spectrophotometric analysis Charge-transfer complexation
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Main Article Content
Spectrophotometric determination of procainamide by the method of charge-transfer complexation with chloranilic acid
Abstract
Spectrophotometric method of analysis of procainamide in its pharmaceutical dosage form by charge-transfer complexation using chloranilic acid was investigated. This investigation was designed to show that charge-transfer complexation can be a sensitive and accurate technique of assaying procainamide when compared to the official nitrite titration method. Procainamide in the presence of chloranilic acid spontaneously formed a purple complex with lmax at 520 nm. The precision of the technique at various concentrations gave standard deviation range of between 0.70 and 0.85. The limit of detection and quantitation were 1.0 x 10-3 mg/ml and 3.0 x 10-3 mg/ml respectively. The complexation method obeys Beer-Lambert Law over a range of 1.0 mg/ml and 10 mg/ml concentrations and gave recoveries of 98.0 ± RSD 0.76 % as compared with 95.0 ± RSD 0.62 % by the Official sodium nitrite titration method. Statistical comparison of the two methods did not show any significant difference (p>0.05) in their levels of accuracy. The charge-transfer complexation technique is simple, sensitivity and accurate, therefore it can be used alongside the Official method of analysis of procainamide.
Key Words: Procainamide assay; Spectrophotometric analysis; Charge-transfer complexation
Journal of Pharmacy and Bioresources Vol.1(1) 2004: 35-40
Key Words: Procainamide assay; Spectrophotometric analysis; Charge-transfer complexation
Journal of Pharmacy and Bioresources Vol.1(1) 2004: 35-40
