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Published:
Jan 27, 2005Keywords:
Hydralazine hydrochloride Vanillin Hydrazone Colorimetric determination
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Main Article Content
Colorimetric method for the determination of hydralazine hydrochloride in pharmaceutical formulations using vanillin as chromogen
Abstract
A colorimetric method was developed for the quantitative determination of hydralazine hydrochloride in dosage forms. The method was based on the formation of a hydrazone following a coupling reaction between hydralazine and vanillin in acidic condition and the spectrophotometric determination at the absorption maximum. The hydrazone has an absorption maximum (λmax) at 390 nm with a molar absorptivity of 1.429 x 104 L mol –1cm–1. Beer's law was obeyed in the concentration range of 4-20 mg/mL at the wavelength of maximum absorption. The reaction product was stable for at least 24 hours. Overall relative standard deviation and recovery were 1.26% and 100.11% respectively. Excipients used in the pharmaceutical formulation did not interfere with the analysis. The technique offers the advantages of high sensitivity, rapidity and simplicity without the need for extraction, heating or cooling to low temperatures.
Key Words: Hydralazine hydrochloride; Vanillin; Hydrazone; Colorimetric determination.
Journal of Pharmacy and Bioresources Vol.1(1) 2004: 1-6
Key Words: Hydralazine hydrochloride; Vanillin; Hydrazone; Colorimetric determination.
Journal of Pharmacy and Bioresources Vol.1(1) 2004: 1-6
