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Published:
Dec 2, 2015DOI:
10.4314/jpb.v12i2.8Keywords:
Article Details
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Main Article Content
Effect of paracetamol on the plasma protein binding of quinine sulphate
Abstract
The co-administration of antimalarial and antipyretic drugs is a common practice in the treatment of malaria. Paracetamol, which is a majorly used antipyretic drug, has proved useful in the management of some common feverish effects such as pains and headache associated with most antimalarial drugs. This study was done to investigate if the co-administration of quinine and paracetamol could have an effect on the protein binding of quinine. Exactly 5ml of plasma contained in a dialysis sack was placed into 20ml of varying concentrations (2 -10µg/ml) of test sample at 37oC. 2ml of the dialysate was basified with 20%NaOH after 8 hours above equilibrium time of 5 – 7 hours, and then extracted with diethyl ether and 0.1M HCl. The absorbance of the resultant solutions was taken at the wavelength of 350nm. The results showed that the albumin concentration in plasma was 4.04 ± 0.03% and the time at which equilibrium was attained was between 5 and 7 hours. The degrees of binding of quinine to plasma proteins for the concentration between 2 – 10 µg/ml in the absence and presence of paracetamol were respectively, 75.5 – 62.7% and 64.7 – 56.0%. A statistically significant decrease (P < 0.05) was observed. From this study, paracetamol has been shown to decrease the protein binding of quinine to both plasma proteins and 4.04% albumin.
Keywords: Co-administration; Equilibrium dialysis; Concentration; Quinine; Paracetamol
