Antibacterial activity of Globimetulla browni (Mistletoe) leaf extract against four clinical bacterial isolates

Abstract

The antibacterial properties of ethanolic extracts of Globimetulla browni (Mistletoe) against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae were investigated using agar well diffusion method. The ethanolic extract of Globimetulla browni contained appreciable amount of tannins, moderate amount of alkaloids, cardiac glycosides, and saponins, trace amounts of steroids and total absence of flavonoids. At both 1000 mg/ml and 100 mg/ml concentrations, the antibacterial activity of the extract revealed high zones of inhibition against Staphylococcus aureus while the least zone of inhibition was observed against Klebsiella pneumoniae. The minimum inhibitory concentration (MIC) was determined using spread plate method. The MIC was observed at the concentration of 500 mg/ml against Staphylococcus aureus and Escherichia coli. At 1000 mg/ml, the MIC was observed against Pseudomonas aeruginosa, but the extract showed no effects against Klebsiella pneumoniae, while the minimum bactericidal concentration (MBC) was observed at the concentration of 500 μg/ml against Staphylococus aureus.