Isolation and characterization of lupeol and stigmasterol from methanol root extract of Combretum hypopolinum (diels.) Okafor(Combretaceae)

Abstract

Lupeol (a pentacyclic triterpenoid) and stigmasterol were isolated from the ethyl acetate fraction of the methanol root extract of Combretum hypopilinum, a plant known to have a wide range of medicinal uses in traditional medicine such as pain, infertility and tuberculosis treatment. The chromatographic analysis of the ethyl acetate fraction of the crude methanol extract led to the isolation of lupeol and stigmasterol which were elucidated and characterized using their IR,1HNMR,and ¹³C NMR spectral data in comparison with literature.