Interfering microbes in virological processes and their antimicrobial resistance pattern

Abstract

Contamination of allantoic fluid obtained from chicken embroynated eggs is the presence of other microbes other than the organism of interest. This study investigated the antimicrobial susceptibility profile of bacteria contaminants of virus isolation procedures with a view to highlighting their diagnostic and public health risk. A total of 100 allontoic fluid samples that grew on blood agar and 50 environmental samples from different laboratory work surfaces were collected and inoculated onto MacConkey and Blood Agar and incubated at 37°C for 24 hrs. Discrete colonies were examined and characterized by standard microbiological protocols. Pseudomonas aeruginosa, Bacillus spp, Klebsiella spp, E. coli, Staphylococcus aureus and Yeast cells were the predominant microbes isolated. However, Pseudomonas aeruginosa were isolated from allantoic fluid samples in 26 cases and 6 from environmental samples. Susceptibility studies were performed according to CLSI standard. Chloramphenicol, ciprofloxacin and ceftriaxone, showed a slight susceptibility. However, penicillin, gentamicin, tetracycline, erythromycin and streptomycin showed different levels of resistance to the organisms tested. In addition, Bacillus spps were most susceptible to chloramphenicol, ciprofloxacin, ceftriaxone, tetracycline and erythromycin of all other bacteria isolates. This study reports that Bacillus spp, Klebsiella spp, P. aeruginosa, E. coli, Providencia stuartii, Yersinia enterolitica and yeast cells have hemagglutinating properties. Occurrence of antimicrobial resistant organisms in tissues and the environment observed in this study shows the extent of the challenges of AMR.