Evaluation of microparticles based on chitosan and sodium alginate blend for enhanced delivery of ciprofloxacin against plasmid-mediated drug resistance

Abstract

The study seeks to evaluate microparticles based on chitosan and sodium alginate blend for enhanced delivery of ciprofloxacin in plasmid-mediated drug resistance. Microparticles were prepared using the ionotropic-gelation method. Three different ratios were prepared for optimization. The microparticles were optimized based on encapsulation efficiency, in-vitro drug release, pH stability and bacterial susceptibility studies. The encapsulation efficiency of the three batches was higher than 40 %. The formulations showed an extended release profile over 8 h and reached a peak of about 30 % release at the 6 h. The pH of the particles was stable over a period of 6 d. The microparticles containing only 0.075 mg of ciprofloxacin dropped in an agar well plate inoculated with E. coli Flac Tn3 gave an IZD of ≥ 6 mm. The microparticulate blend of chitosan and sodium alginate holds good potentials for ciprofloxacin delivery against plasmid-mediated drug resistance.

Keywords: microparticles, ciprofloxacin, chitosan, sodium alginate, plasmid, resistance