Development and validation of RP-HPLC method for identification and quantification of quercetin in chloroform extract of Guiera senegalensis leaf

Abstract

Guiera senegalensis (Sabara in Hausa) is a shrub with numerous  medicinal values attributed to its secondary metabolites e.g. quercetin. The aim of this work is to develop and validate an RP-HPLC method for the identification and quantification quercetin in Guiera senegalensis. After several trials, the optimized condition comprises of a C18 column (250 x 4.6 mm), ternary mobile phase (Methanol: Water: acetonitrile in 55:5:45) containing 0.1 % orthophosphoric acid as additive, isocratic elution mode, 255 nm detection wavelength, flow rate of 1.2 mL/min, 10 μl injection volume, a runtime of 7 minutes and at ambient temperature. Quercetin was found to be eluted at 2.2 minute. The calibration curve was linear within the range of 10-50 μg/mL (r2 = 0.999) and was sensitive as indicated by a lower LOQ (3.15 μg/mL). The method was found to be precise, accurate and robust as indicated by their respective values which are within the acceptable limits.

Key Words: Guiera senegalensis, Quercetin, RP-HPLC, isocratic elution, ternary mobile phase.