Histological and biochemical evaluation of Juglans regia L (Walnut) as potential remedial properties on the lungs and liver tissues of albino rats pre-treated with methotrexate

Abstract

A multipurpose tree species known for its nutritional, medicinal, and commercial worth is Juglans regia Linn. Higher dosages of the multipurpose medicine methotrexate can be hazardous to the liver. It is a widely used anti-rheumatic therapy. The liver and lungs of Wistar albino rats that had been pretreated with methotrexate were studied to see what effect Juglans regia Linn had and what benefits it might have. Five groups (control, negative, positive, and counter groups) of twenty-five Wistar albino rats were used in the study design. The assessment included histological alterations and hepatic biomarkers (globulin, albumin, TP, CB, ALP, and ALT)by which method. To evaluate the obtained data, SPSS version 20, a statistical programme for social sciences, was utilised. When comparing the groups, the biomarkers did not reveal any discernible differences at p<0.05. The experimental animals in group B showed modest structural abnormalities in their liver tissue and an inflammatory effect on their lung tissue in histopathological findings. On the hepatic and pulmonary tissues, groups A and C showed no effects. Notwithstanding, there were minor structural alterations noted in the liver tissue of group D. Since the liver and lungs in group E showed no reaction to the medication, it is possible that the extract could offset the drug's effects at greater dosages.This study suggests that Juglan regia Linn could amelioratethe liver and lung effect of methotrexate in Wistar albino rats.