In-Vitro Evaluation of Antibacterial Activity of Congronema Latifolium (UTASI)

Abstract

Experiments were conducted to evaluate the anti-bacterial activity of Congronemalatifolium, a local plant used in ethno medicine among the local populations in Nigeria. The methanolic extract of Congronema latifolium leaf meal (CLLM) was tested against Staphylococcus aureus, Salmonella spp., Pseudomonas aeruginosa, E. coli, and Klebsiella spp. Determination of anti-bacterial activity was carried out using the procedures of minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and the zone of inhibition test (ZIT). P. aeruginosa and E. coli were the most sensitive to CLLM extract, with MIC of 15.625, MBC of 31.25mg/ml, and a ZIT score of 1.4 and 1.5cm, respectively, while the other test organisms exhibited minimal sensitivity. The results suggest that themethanolic extract of CLLM could be used as an organic antibiotic against some enteric organisms of monogastric animals.