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Published:
Jul 29, 2024DOI:
10.4314/jnoa.v26i1.5Keywords:
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Main Article Content
Identification of Contact lens bacterial Isolates from Students of University of Benin, Nigeria, Using 16S rRNA Gene Sequence Analysis
Abstract
Purpose: Molecular phylogenetic analyses have proven beneficial in overcoming some limitations of traditional phenotypic procedures for the detection and characterization of bacterial isolates. The aim of this study was to identify bacterial isolates from hydrogel (soft) contact lenses worn by selected students of the University of Benin, Nigeria, using 16S rRNA gene analysis.
Methods: Eleven bacterial isolates from the worn hydrogel lenses, which remained unidentified with the traditional phenotypic techniques, were transferred to the molecular biology section of the Labor Medical and Research Laboratory for possible identification by phylogenetic analysis, to have a full range of bacteria considered as normal flora of the eyes. Bacterial genomic DNA was extracted with the ZymoBIOMICS DNA Mini Kit (Zymo Research Corp., Irvin, CA, USA), amplified with polymerase chain reaction (PCR), and the amplicons were sequenced with the ABI3500xL Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). The nucleotide sequences were assembled and deposited in the GenBank database for alignment with the prototype strains.
Results: The bacterial isolates identified were Burkholderia cenocepacia GIMC 4560: BCN122, Cupriavidus pauculus KPS 201, and Comamonas testosteroni M.pstv. 4.2, Acinetobacter calcoaceticus NBRC 13006, Achromobacter denitrificans SW2, Bacillus cereus FORC_048, Staphylococcus saprophyticus Marseille-P541, Burkholderia cenocepacia GIMC 4560: Bcn122, Acinetobacter calcoaceticus 7.3, and Comamonas sp. 6.1 and Acinetobacter sp. pp2a.
Conclusion: The 16S rRNA gene sequence analysis was able to identify 82% of the isolates at the strain level and 18% at the genus level.
