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In vitro investigation of the antitrypanosomal potentials of Manganese on the lipids of Trypanosoma brucei brucei
Abstract
Background: Trypanosomiasis is one of the major health conditions affecting mankind. This study examined the influence of manganese on the lipid profile of Trypanosoma brucei brucei with the hope that this will form the basis for the formulation of a potent chemotherapeutic agent against trypanosomiasis.
Methodology: Bloodstream T. b. Brucei grown in rats was separated without any host contaminants using DEAE cellulose chromatography and dry weight of the parasites determined. One mililitre each of phosphate saline glucose buffer (containing 100mg parasites) was added into 8 test tubes and each incubated with graded concentrations(1.0 x 10-1M, 1.0 x 10-2M, 1.0 x 10-3M, 1.0 x 10-4M, 1.0 x 10-5M, 1.0 x 10-6M, 1.0 x 10-7M and 1.0 x 10-8M respectively) of MnCl2 solution. A 9th test tube (carrying 100mg T. b. brucei in 1ml PSG) served as control. Total lipid of the parasites in each tube was extracted and profile of the fractions assayed to investigate the impact of Manganese.
Results: Manganese caused a general increase in total lipid profile of the parasites by 5.8%. The effect of treatment with MnCl2 on the profile of total neutral and polar lipid fractions of T. b.brucei varied. In polar lipids, increase ranged between 1.5% and 14.5% while increase in neutral lipids ranged between 10% and 35%. Furthermore, the metal more varied effect on individual lipid components (Lypophosphatidyl choline, Phosphatidyl choline, Phosphatidyl serine, Phosphatidyl ethanolamine, sterol,Monoacyl glycerides, Free fatty acids, Diacyl glycerides and Triacyl glycerol).
Conclusion: This study has shown that Mn caused significant changes in the levels of various lipid fractions in T. b. Brucei suggesting that it can be beneficial in chemotherapy.
Methodology: Bloodstream T. b. Brucei grown in rats was separated without any host contaminants using DEAE cellulose chromatography and dry weight of the parasites determined. One mililitre each of phosphate saline glucose buffer (containing 100mg parasites) was added into 8 test tubes and each incubated with graded concentrations(1.0 x 10-1M, 1.0 x 10-2M, 1.0 x 10-3M, 1.0 x 10-4M, 1.0 x 10-5M, 1.0 x 10-6M, 1.0 x 10-7M and 1.0 x 10-8M respectively) of MnCl2 solution. A 9th test tube (carrying 100mg T. b. brucei in 1ml PSG) served as control. Total lipid of the parasites in each tube was extracted and profile of the fractions assayed to investigate the impact of Manganese.
Results: Manganese caused a general increase in total lipid profile of the parasites by 5.8%. The effect of treatment with MnCl2 on the profile of total neutral and polar lipid fractions of T. b.brucei varied. In polar lipids, increase ranged between 1.5% and 14.5% while increase in neutral lipids ranged between 10% and 35%. Furthermore, the metal more varied effect on individual lipid components (Lypophosphatidyl choline, Phosphatidyl choline, Phosphatidyl serine, Phosphatidyl ethanolamine, sterol,Monoacyl glycerides, Free fatty acids, Diacyl glycerides and Triacyl glycerol).
Conclusion: This study has shown that Mn caused significant changes in the levels of various lipid fractions in T. b. Brucei suggesting that it can be beneficial in chemotherapy.
