Assessment of biochemical and haematological changes that occur in blood stored with CPDA-1 as an anticoagulant in a Tertiary Hospital in Nigeria

Abstract

Transfused blood must be of required quality to meet body metabolic requirements. This study monitored variations in biochemical and hematological parameters following blood transfusion. Twenty blood donors age range 18-55yr were recruited. About 450mls blood was drawn into 0 blood bag containing citrate phosphate dextrose adenine (CPDA-1) and stored at 2-8 C in blood bank. Ten millilitres blood was withdrawn on different days, centrifuged to obtain the plasma which was analysed using standard methods.
Result shows a significant decrease (p<0.05) in plasma antioxidants, sodium, chloride, urea, lipid, protein, alkaline phosphatase, haematological parameters; and significant increase (p<0.05) in potassium, malondaaldehyde, bilirubin, and transaminases on day 35 when compared to values at day 0. In conclusion, storing of blood using CPDA-1 as an anticoagulant is associated with variations in biochemical and hematological parameters which were noted to occur after the seventh day of storage. Blood for transfusion is thus best used on or before the seventh day of donation.

Key words: Transfusion, blood storage, anticoagulant, biochemical parameters, haematological parameters.