Prediction of Methotrexate toxicity in Algerian Rheumatoid Arthritis Patients: Implication of GGH rs11545078 polymorphism

Abstract

Abstract
Objectives - The aim of this study is to determine the impact of the gamma glutamyl
hydrolase (GGH) c.452C>T (dbSNP Id: rs 11545078) polymorphism on the Methotrexate
(MTX) adverse drug reactions (ADRs) and on MTX gastro intestinal toxicity (GIT) in rheumatoid arthritis (RA) patients.
Materials and methods - Sixty-one patients with RA are enrolled in this study. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Data were analyzed by χ2.
Results - Our findings suggest that the frequencies distributions of alleles/genotypes of
this polymorphism were similar in both groups with and without ADRs. However, there
is a significant distribution of GGH 452TT (p=0.03) and GGH 452CT+452TT (p=0.02) genotypes between the groups with and without GIT. The same correlation was found for
GGH 452T allele (p=0.008).
Conclusion - We have shown for the first time in the West Algerian population that the
GGH c.452C>T polymorphism influences the MTX gastro-intestinal toxicity