Identification and optimization of lipase producing bacteria from palm oil contaminated waste

Abstract

Bacteria isolated from semi-solid waste, SS2B1 exhibited a greater zone of clearance, 9mm with higher lipase activity. SS2B1 isolate demonstrated a Gram-positive and rod shape arrangement under microscope observation belong to Bacillus sp based on biochemical characteristics. The effect of carbon source, nitrogen source, medium pH and temperature for the lipase production was studied. The lipase production was maximum (0.1228 μg/ml/min) at pH7, temperature 37⁰C by the lipase producing bacteria SS2B1, Bacillus sp. Increased enzymatic production was obtained when the organisms were cultured in medium supplemented with 1% tryptone and palm oil as substrate with 53.58% optimization process. The results of the present study demonstrate that the Bacillus sp. is ideal for extracellular lipase production at industrial level such as detergent, leather and fine chemical industries.

Keywords: bacteria, lipase; optimization; palm oil contaminated oil; screening; production