Determination of serum interleukin 33 (IL-33) levels in atopic asthma patients using ELISA kits

Abstract

This study compares serum IL-33 levels in atopic asthma (AA) patients and normal controls
to determine serum IL-33 levels. 5 mL of blood were withdrawn from subjects and
centrifuged for 5 min at 2000 rpm to obtain the serum and were analyzed for IL-33 using kits. Data entry and analysis were done using SPSS version 20.0. The results showed serum IL-33 levels were higher in atopic asthma patients than in normal controls. This difference was not statistically significantwith a p-value of 0.218 determined by an independent-T test. The results also showed no significant difference in IL-33 levels according to AA severity with a p-value 0.396 determined by a Kruskal Wallis test. There was no significant difference of
serum IL-33 levels between normal controls and AA patients. The levels of IL-33 and other
predisposing factors showed significant associations in smoking status and occupational
exposure.

Keywords: IL-33; atopic asthma; serum levels; severity; ELISA