High-density lipoprotein attenuates secretion and gene expression of cellular adhesion molecules and proinflammatory cytokines in lipopolysaccharide-stimulated endothelial cells

Abstract

This study was conducted to investigate the effects of high-density lipoprotein (HDL) on
secretion and gene expression of intercellular adhesion molecules-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), endothelial-leukocyte adhesion molecule-1 (E-selectin), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) by human vascular endothelial cells (HUVEC). Lipoploysacharides-stimulated HUVEC was incubated with different concentrations of HDL (20-120 mg/dL) for 16 hours. The levels of all biomarkers were measured by ELISA. Total RNA was extracted and all bioamarkers’ mRNA levels were measured by Taqman real-time polymerase chain reaction assay. Different HDL concentrations reduced the secretion and expressonof all biomarkers except VCAM-1, which may contribute to the prevention and regression of atherosclerosis.

Keywords: HDL; adhesion molecules; cytokines; endothelial cells; lipopolysaccharides.