Phenotypic, molecular and technological characterization of autochthonous lactobacilli strains isolated from cow’s milk and goat of Algerian populations

Abstract

The raw milk is an important reservoir of microbial diversity can have important biotechnological applications, in particular to improve the unique characteristics of dairy products. For this effect, eight strains of autochthonus Lactobacilli have been isolated from goat and cow raw milk of the local Algerian populations (Setifian and Kabyle). The strains were then identified by phenotypic and molecular approaches by amplification and sequencing of 16S rDNA as Lb.casei (C4, C5, V2 and V5), Lb. paracasei (C6) and Lb.plantarum (C7, C8, C10). Virtually all the strains studied, from a technological point of view, produced lactic acid concentrations at or above 0.89g.100 mL^-1. Most strains exhibit a high capacity and are actively producing proteolytic proteases in the stationary phase between 24 and 30 hours. The strains studied can be used in the starter cultures or co-cultures for making cheese.

Keywords: Lactobacillus, identification, acidification, proteolysis.