The effect of allyl isothiocyanate on RNA

Abstract

Allyl isothiocyanate (AITC), finds in cruciferous vegetables. AITC shows bactericidal, fungicidal activities and anticancer activity by inhibition of cell proliferation, cell cycle arrest and apoptosis induction and tumor growth inhibition. In this study, the interaction of Allylisothiocyanate with yeast RNA in aqueous solution at physiological conditions using constant RNA concentration and various molar ratios of AITC/RNA complexes are examine. Fourier transform infrared (FTIR) and uv-visible difference spectroscopic methods are used to determine the AITC binding mode, binding constant and stability of RNA in AITC/RNA complexes in aqueous solution. Spectroscopic evidence shows that AITC interacts with RNA. The external binding of AITC to RNA with overall binding constant of K(AITC-RNA)=7.207×10⁶ M^-1 are also presented. RNA remains in A conformation upon AITC complexation.

Keywords: Allyl isothiocyanate; RNA; FTIR; UV-vis; binding constant; conformation.