Formulation of category-2 hypoglycemic Improved Traditional Medicines from selected Cameroonian medicinal plants: Mangifera indica, Persea americana and Ageratum conyzoides

Abstract

Diabetes mellitus is the fourth leading cause of death worldwide, and constitutes a major public health crisis. Management of the adverse  condition relies mostly on synthetic drugs such as metformin, glibenclamide and insulin. However, treatment with synthetic  drugs is challenged by side effects and high cost especially to patients in low-income countries like Cameroon. This study set out to  formulate Improved Traditional Medicines (ITMs) in the form of capsules from lyophilized aqueous leaf extracts of Ageratum conyzoides,  Mangifera indica and Persea americana, in combination, coded as ITM-1, and leaf extract of Mangifera indica alone, referred to as ITM-2.  Phytochemical profiling of individual extracts was carried out using standard methods, while their antioxidant activity was evaluated in  vitro by the 2,2-diphenyl-1-picryhadrazyl reducing (DPPH) assay and the Ferric (Iron) reducing antioxidant power assay (FRAP). Capsule  formulation was guided by findings from prior Physicochemical and pharmacotechnical analysis of individual extracts and their  combination. Safety studies were carried out both in vitro (cytotoxicity testing in Vero cells); and in vivo (acute toxicity tests using the  mouse model). Preliminary evaluation of the antidiabetic potential of the formulated ITMs was achieved through determination of their  acute hypoglycemic and antihyperglycemic properties in Wistar rats (Oral Glucose Tolerance Test). Major classes of phytochemicals detected were alkaloids, phenols, tannins, flavonoids, anthocyanins, tri-terpenes and anthraquinones. The DPPH and FRAP assays showed  dose-dependent antioxidant activity for ITM-1 and ITM-2. Both ITMs showed no toxic effects, be it in vitro, (Cytotoxic  Concentration 50% - CC50>1000 µg/mL in cells) or in vivo. At the dose of 25 mg/Kg, both ITMs exhibited significant hypoglycemic effects  in Wistar rats. The ITM-2 capsules completely suppressed post-prandial glucose peak in rats as compared to the negative control (distilled  water), ITM-1 capsule was able to cause restoration of the glucose levels to normal levels after 120 minutes