Bio-treatment of phosphate from synthetic wastewater using Pseudomonas sp YLW-7

Abstract

In this study, the efficient phosphate utilizing isolates were used to remove phosphate from synthetic phosphate wastewater was tested using batch scale process. Hence the objective of the present study was to examine the efficiency of bacterial species individually for the removal of phosphate from synthetic phosphate wastewater. The most efficient phosphate reducers were isolated and screened from eutrophic lake water samples. The total heterotrophic bacterial analysis of the samples showed the presence of about 22 phosphate reducers. Among them, Pseudomonas sp YLW-7 were found to be efficient in phosphate reduction based on the maximum phosphate ultization which was observed by plate screening method using the minimum inhibitory concentration (MIC) test. The effect of carbon sources (glucose, starch, sucrose and lactose) at 0.5% on the removal of phosphate by Pseudomonas sp YLW7 was estimated. The maximum growth of Pseudomonas sp YLW7 was observed to be 0.9886 OD in glucose followed by starch (0.9456 OD), Sucrose (0.9095 OD) and lactose (0.8407 OD). The pH change in culture medium after 72 h treatment was found to be 6.0 in glucose, starch, lactose sources and 5.8 in sucrose carbon source. The phosphate removal was observed to be maximum of 68 % in synthetic phosphate wastewater with glucose carbon source followed by starch (66%), sucrose (65%) and lactose (62%) after 72 h at neutral pH (7.0± 2) by Pseudomonas sp YLW-7. Thus the Pseudomonas sp YLW-7 may use the contaminants as nutrients source and might be used in the remediation of phosphate contaminated environments.