Phytochemical Characteristics, in-Vitro and in-Vivo Antioxidant Potentials of Ethyl acetate Fraction from Apple-ring acacia (Faidherbia albida (Delile) A. Chev.) Leaves Extract on Albino Rats

Abstract

The objective of this paper as to investigate the phytochemical characteristics, in-vitro and in-vivo antioxidant potentials of ethyl acetate fraction from apple-ring acacia (Faidherbia albida (Delile) A. Chev.) Leaves Extract on Albino Rats using appropriate standard procedures. Phytochemical examination identified alkaloids, steroids, saponins, glycosides, phenols, and tannins in both extracts. The antioxidant potential evaluated using the Ferric Reducing Antioxidant Power (FRAP) assay, show ethyl acetate fraction exhibited dose-dependent activity akin to that of vitamin E (34.69, 40.28, 70.26). In-vivo investigations assessed the impact of the extracts on oxidative stress indicators, comprising malondialdehyde (MDA) [102.77±2.99], glutathione (GSH) [110.28±0.39], catalase (CAT) [1.93±0.12], superoxide dismutase (SOD) [3.58±0.14], and concentrations of vitamins A [61.02±0.48], C [555.99±1.66], and E [71.24±0.49] respectively. The ethyl acetate fraction dramatically (P<0.05) decreased MDA levels, signifying diminished lipid peroxidation, while preserving GSH, CAT, and SOD levels near baseline. The results indicate a significant antioxidant capacity of the ethyl acetate fraction, offering defense against oxidative The research indicates that the ethyl acetate fraction of Faidherbia albida leaf exhibits significant antioxidant activity, positioning it as a viable candidate for medicinal use in managing oxidative stress.